Agilent 8453 UV-Visible Spectrophotometer

Agilent 8453 UV-Visible Spectrophotometer

**Unit was tested to specifications. Unit was confirmed to have a working bulb and accurate readings. Unit has a few signs of cosmetic wear on the exterior portions of the machine as well as on top of the unit... please see the photos for more details. 

The Agilent 8453 Spectrophotometer is an essential component in UV-Visible systems. Routine and occasional users can take advantage of the diode-array features including fast scanning, excellent sensitivity, and virtually absolute wavelength reproducibility.

Featuring exceptional ruggedness and reliability, this Agilent spectrophotometer is easy to use with a line power indicator, a status indicator and other push buttons. This equipment uses a photodiode array (PDA) which highly reliable. Simultaneous measurements of the complete light spectrum take less than a second using this array. Effective data analysis and archival with the use of data analysis software ensures compliance with good laboratory practices (GLP) and pharmacopoeia requirements.

Offering minimum maintenance and an open sample area, the Agilent 8453 Diode Array Spectrophotometer has excellent optical performance. Unlike conventional instruments, the Agilent 8453 is not weighed down by ambient stray light.

Instantly measure sample absorbance across the complete mid-UV-visible spectrum.

Typical scanning spectrophotometers give the user a choice: speed or breadth.  Opting for speed, you focus on a particular wavelength of interest (e.g., 280nm for protein concentration, 600nm for cell count), blind to the rest of the spectrum and the interesting and useful data it might contain.  Opting for breadth, scanning the entire spectrum can take half a minute or more, often too slow to accurately study a dynamic sample.
With its photodiode-array-based sensor system and only a single moving part, the 8453 simultaneously reads the entire available spectrum, combining speed and breadth with high accuracy and sensitivity. Not only can you look at the absorbance of your sample at standard measurement wavelengths, but you can also check for unexpected absorbance elsewhere along the spectrum so as to discern impurities, contaminants, and other aberrations.

• Spectra are collected in 0.1 seconds, with a resulting sampling frequency limit of 10 Hz, allowing constant and systematic monitoring of samples: particularly useful for dynamic samples.
• Wavelength range: 190-850nm
• Sample must be in solution.
• Peltier unit attached to instrument for precise temperature control.
• Open sample compartment for quick sample changing, mixing, reagent addition, etc.
• Uses a tungsten lamp for visible wavelengths and a deuterium lamp for UV.
• Because the diffraction grating is opposite the sample from the light sources, stray light entering the sample compartment is not an issue.