Agilent G3100A OFFGEL Fractionator Electrophoresis CLEARANCE! As-Is

Agilent G3100A OFFGEL Fractionator Electrophoresis System

***This instrument has been tested to factory specifications. Buyer can expect a working machine and 30-day warranty. Unit has slight wear from normal use.  See photos for details.

Easy integration into any protein analysis workflow. Whether your task is biomarker
discovery, protein identification or purification of functional proteins or peptides, the Agilent 3100 OFFGEL Fractionator fits into any protein analysis workflow. Integration of the OFFGEL system is easy and ultimately increases sensitivity of subsequent MS detection.

Benefits:

• Pi-based OFFGEL fractionation with liquid-phase recovery for easy
  transfer to downstream techniques such as LC/MS
• Reproducible fractionation with resolution as low as 0.1 pH for maximum MS sensitivity
• Compatibility with up- or downstream techniques such as immunodepleting, LC/MS or gel-based analysis for maximum flexibility
• Pi values obtained act as additional validation parameters of MS hits and can be used to search for peptides with charged posttranslational modifications (PTMs)
• All additives can easily be removed after fractionation to avoid interference with nano-electrospray and MS detection.
• Fractionation of up to 16 samples in parallel on two trays (eight samples each) for highest throughput
• Broad loading capacity from 50 µg to 5 mg of sample for analytical applications or maximum enrichment of low abundance proteins
• OFFGEL mode as well as conventional isoelectric focusing (IEF) based on immobilized pH gradient (IPG) gels.

The Agilent 3100 OFFGEL Fractionator performs isoelectric focusing of proteins or peptides in immobilized pH gradient (IPG) gel strips. The technique applied by the 3100 OFFGEL Fractionator differs from conventional gel electrophoresis in that sample components are recovered from liquid phase. This makes the technology.
directly compatible with liquid-phase workflows, for example LC-MS.

Features:

• Easy setup with preinstalled methods for OFFGEL and conventional IPG-based IEF modes
• State-of-the-art user interface for intuitive instrument setup and control and online monitoring of all key parameters that affect fractionation quality.
• Independent high-voltage power supplies allow two different methods or sample sets to be run simultaneously.
• Automated storage of up to 1200 data files and easy export of run parameters.
• Monitoring of all operations and error events in self-updating logbook
• Cover design allows fractionation of light sensitive protein samples.

Manufacturer's Specifications: