BMG Labtech PHERAstar FSX HTS Microplate Reader

BMG Labtech PHERAstar FSX HTS Microplate Reader

Name: BMG Labtech PHERAstar FSX HTS Microplate Reader
Brand: REUZEit Inc
SKU: 3374193
Price: 40000 USD
Availability: OutOfStock

sku: 3374193

Out Of Stock

Condition : Pre-owned

Warranty : 30-Day Warranty, 100% Parts and Labor

Region : US

$40,000.00

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Make BMG LABTECH
Hertz 50Hz/60Hz
Model PHERAstar FSX
Serial 472-1058
Weight 148
Voltage 120V/220V
Working Working
Includes Power Cord
Warranty 30-Day Warranty, 100% Parts and Labor
Dimensions 27×25×25 in
Shipping Type FedEx Ground
Item Condition Pre-owned

BMG Labtech PHERAstar FSX HTS Microplate Reader

***Preowned instrument with very low prior usage. We tested the unit as far as we could and there are no known issues. Software and license are not included. Buyer can expect a working machine with 30-day warranty. See photos for details.

No longer worry about setting or tuning different wavelengths for your absorbance assays. The spectrometer will capture full-absorbance data at a resolution of 1 nm for all wavelengths from 220 to 1000 nm in less than one second per well. Alternatively, up to eight discrete wavelengths can be collected at the same time.

The spectrometer permits absorbance experiments that were previously not possible. Due to the ultra-fast speed of spectral capture, time-course experiments can be monitored for wavelength shifts with 0.2-second resolution. Absorbance shifts can be monitored in real-time as an assay goes to completion. The spectrometer technology allows users to focus on developing assays and expanding capabilities without worrying about running instrumentation.

BMG LABTECH is the only microplate reader company in the world offering spectrometer technology in a multi-detection microplate reader.

The PHERAstar^® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen^® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.

Absorbance
Fluorescence intensity (incl. FRET)
Luminescence (incl. BRET)
Fluorescence polarization
AlphaScreen
Nephelometry
Time-resolved fluorescence
TR-FRET

No longer worry about installing filters and dichroics: the PHERAstar FSX comes with easy-to-handle, assay-optimized Optic Modules which contain all application-specific filters, mirrors, dichroics and/or polarizers, and are automatically recognised by the plate reader. In addition, the PHERAstar FSX is equipped with four matched and assay-optimized photomultiplier tubes (PMTs) which are automatically selected for the relevant detection mode. You can also measure assays with two emission wavelengths at the same time thanks to the Simultaneous Dual Emission.

For HTS automation purposes, the PHERAstar FSX can be easily integrated into automated systems, and offers improved robotic integration capabilities and multi-user control.

Features

The most sensitive reader in fluorescence intensity and polarization
The only plate reader with 5 assay-dedicated detectors
Simultaneous Dual Emission, including for Alpha Technology
9 decades luminescence dynamic range
Dedicated AlphaScreen^®/AlphaLISA^®/AlphaPlex™ laser
New generation TRF-laser
Top and bottom focal height adjustment
High-precision injectors with simultaneous reagent injection and detection
High-speed absorbance measurements
Advanced Assay Stability system

Technical Specifications:

Detection Modes

Fluorescence intensity - incl. FRET
Fluorescence polarization
AlphaScreen^®/AlphaLISA^®/AlphaPlex™Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra

Measurement Modes

Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Simultaneous Dual Emission
Spectral scanning (absorbance)
Real-time ratiometric measurements
Well scanning

Microplate Formats

Up to 3456-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier: Robot compatible

Light Sources

High energy xenon flash lamp
Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex™Dedicated laser for TRF and TR-FRET

Detectors

Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes
CCD spectrometer

Wavelength Selection

Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application)
For top and bottom reading
UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec/well

Spectral Range

Filters
230 - 750 nm or 230 - 900 nm for FI, FP
230 - 750 nm for LUM
230 - 900 nm for TRF

Spectrometer
220 - 1000 nm for ABS; wavelength precision: ≤ 0.5 nm

Sensitivity

FI
(top)
< 0.15 pM FITC (384sv black, 20 μL)
< 0.5 pM FITC (1536 black, 8 μL)

FI
(bottom)
<1.0 pM FITC (384g black, 50 μL)

FP
< 0.5 mP SD at 1 nM FITC (384sv black, 20 μL)
< 1.5 mP SD at 1 nM FITC (1536 black, 8 μL)

TRF with laser.
< 5 fM europium (384, 80 μL)
< 15 fM europium (1536, 8 μL)

HTRF^®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 1100% (High Calibrator)
Delta F > 25% (Low Calibrator)

LUM
< 0.4 pM (< 8 amol/well ATP, 384sv white, 20 μL)
Dynamic Range: 9 decades

AlphaScreen^®with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv white, 20 μL)

ABS with spectrometer
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Linearity: ≤ 0.8% at 2.0 OD

Read Times

Flying mode (1 flash)
14 s (384)
27 s (1536)

10 flashes
38 s (384)
1 min 52 s (1536)

50 flashes
1 min 29 s (384)
5 min 18 s (1536)

Shaking: Linear, orbital, and double-orbital with user-definable time and speed

Incubation: +5 °C above ambient up to 45 °C

The upper heating plate of the incubation chamber operates at +0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer

With Advanced Assay Stability (AAS) system: from 18 - 45°C

Software

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Dimensions

Width: 45 cm, depth: 51 cm, height: 47 cm; weight: 49 kg

With AAS system: width: 48 cm, depth: 68 cm, height: 47 cm; weight: 62 kg

Optical Path Guides

Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

Z-Adjustment

Automatic focal height adjustment (0.1 mm resolution)

Reagent Injection

Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL, optionally up to 2 mL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing

Integrated Barcode Reader: Up to three integrated barcode readers

Power Requirements

100 or 115 or 230 V, 50/60 Hz, auto ranging

Power Consumption

Normal operating conditions: 50 W
Idle State: 20 W
AAS-option: + 30 W
Switched off: 0 W max. 300 VA

Optional Accessories

LVis Plate

Sample Capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples

Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates

THERMOstar

Microplate incubator and shaker

Stacker

Magazines for up to 50 plates - continuous loading feature

Upgrades

Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen^® P-Tyr-100 assay kit, PerkinElmer, #6760620C
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates