Millepore Mobius 200 Liter Single Use Mixing System, Jacketed Stainless

Millipore Mobius 200 Liter Single Use Mixing System, Jacketed Stainless

**this unit is preowned and in very good condition. Buyer can expect a working system that comes with a 30 day warranty. See pictures for details. FOB: Franklin, MA

Mobius® Power MIX 200 is a 200 liter high performance,
single-use mixing system targeted for small scale
mixing in biopharmaceutical product/process
development and manufacturing. Leveraging the
proven technology of magnetically coupled NovAseptic®
mixers, traditionally used with stainless steel tanks,
the Mobius® Power MIX platform offers solutions for
a wide range of mixing applications, including high
concentration buffers and hard-to-mix cell culture
media. This application note presents performance data
from the Mobius® Power MIX 200 for mixing of these
sinking and floating powders. A characterization map of
liquid-liquid mixing for a range of volumes and speeds
is also included.
Figure 1. Power MIX 200 stainless steel and plastic carriers
Mixing Type Final Product Mixing Time
Liquid-Liquid NaCl solution 25 seconds
Sinking Powder, low concentration 1X DPBS Buffer 2 minutes
Sinking Powder, high concentration 1.5M NaCl 9 minutes
Floating Powder, basal medium DMEM 7 minutes
Floating Powder, chemically defined medium MilliporeSigma Ex-Cell® Advanced™ Medium 14 minutes
Floating Powder, chemically defined medium Custom MilliporeSigma CHO Medium 30 minutes
Introduction
Mixing of buffers (sinking powders) and media (floating
powders), presents substantial challenges in getting
good dispersion and dissolution of particles. The
creation of a vortex and abundant surface movement
in the Mobius® Power MIX 200 is key to the success
in these processes. The axial and radial flow patterns
allow for quick distribution of sinking powders,
minimizing settling at the bottom of the vessel.
Floating powders are drawn into the vortex, allowing
for effective wetting and distribution throughout the
entire vessel volume. With impeller speed up to 380
rpm, complete mixing for even the most difficult
powders can be achieved in less than 30 minutes.
Quantification of mixing time is traditionally
accomplished by tracing the response of pH and/
or conductivity, along with visual observations of
powder dissolution. In these trials, conductivity and
pH sensors are in two locations within the vessel; in
the probe port at minimum volume and at surface
of the liquid. Video records of the mixing process
provides the visual data. In addition to conductivity
and pH measurements, an FBRM® (Focused Beam
Reflectance Measurement) probe is used to track the
distribution of particles over time. The FBRM® probe
from Mettler Toledo® uses a focused laser beam directly
into the process, determining the backscatter of light
to measure and count particles. Analysis of the trace
curves of conductivity and particle distribution to find
stability is used to determine mixing time, matching
this measured response to the visual data