Thermo Fisher Scientific LTQ Orbitrap Elite Velos Pro with ETD

Thermo Fisher Scientific LTQ Orbitrap Elite Velos Pro with ETD

 **This unit is preowned with minimal wear from normal use. System will need to be validated/calibrated to ensure accurate results.  Buyer can expect everything pictured with 30-day warranty. See photos for details.

Includes

• E2M30 Pump code: A374-+15-903 w/oil Mist Filter EMF20. Code: A46229000
• RV3 Finnigan Pump code: R14000004 w/oil Mist Filter EMF20. Code: A46229000
• Velos Pro
• Orbitrap Elite
• ETD

Thermo Scientific Orbitrap Elite

Analyze demanding proteomics, metabolomics, lipidomics and metabolism samples in greater depth using the Thermo Scientific Orbitrap Elite Hybrid Ion Trap-Orbitrap Mass Spectrometer. This instrument combines a high-field Thermo Scientific Orbitrap mass analyzer and Thermo Scientific Velos Pro ion trap technology to deliver very high resolution, speed, sensitivity and complementary fragmentation information.

Thermo Scientific Velos Pro

• Resolving power up to 100,000 FWHM and mass accuracy better than 1ppm RMS provide confidence when analyzing complex samples
• Multiple fragmentation techniques—CID, HCD, and optionally ETD—offer versatility and facilitate structural characterization
• Fast scanning and parallel MS and MSn analysis speeds analyses
• Ion optics with novel neutral-blocking technology increase robustness
• Collision cell with axial field gradient improves quantitative results
• High mass resolution and mass accuracy enable high-confidence molecular weight determination of intact proteins, peptide identification in complex samples and analysis of isobaric species
• High sensitivity increases detection of low-abundance peptides and proteins
• Short duty cycle increases sequence coverage within in a single experiment
• Stability, mass accuracy and wide dynamic facilitate label-free quantification
• Mass resolution and mass accuracy distinguish between heavy and light peptide ion pairs and interferences in SILAC experiments
• HCD cell with axial field improves quantification of isobarically labeled peptides
• Optional electron-transfer dissociation (ETD) generates complementary information for P analyses and de novo sequencing

Thermo Scientific ETD

Achieve better analysis of proteins and their post-translational modifications (PTMs) with the Thermo Scientific™ Electron Transfer Dissociation (ETD) option. Unlike CID, which cleaves weakly bound modifications off the peptide backbone, ETD induces fragmentation along the peptide backbone in a sequence-independent manner, leaving labile modifications linked to the peptide chain. This greatly simplifies identification of modification sites. ETD produces primarily c- and z-type fragment ions that complement the b- and y-type ions produced by CID, increasing sequence coverage and protein IDs.

• ETD preserves labile PTMs, allowing straightforward identification of the peptide sequence and the site of modification
• For top-down analysis, ETD can be combined with proton transfer reaction (PTR) which dramatically simplifies spectra from intact proteins
• For workflows that include low-mass isobaric mass tags, ETD can be combined with pulsed-Q dissociation (PQD) which eliminates the normal low-mass cutoff
• ETD can be added to most Thermo Scientific LTQ-series ion trap and hybrid ion trap-Orbitrap mass spectrometers
• Proteome Discoverer software includes the proprietary Z-Core database search algorithm specifically optimized for ETD spectra
• Proteome Discoverer software can easily merge complementary CID and ETD results to maximize protein sequence coverage

The Edwards E2M30 Rotary Vane Vacuum Pump offers excellent ultimate pumping speed while providing quiet operation. With outstanding vapor handling capabilities, the E2M30 is a reliable and versatile 208V vacuum pump.

• Large, clearly marked, easily accessible controls
• Operate at quiet 48dBA
• Easy to use and service (require no special tools)
• Fast-acting inlet valve prevents oil and gas suck-back in the event of power or oil pressure loss
• NW25 inlet/outlet connections 

High vacuum or high throughput from the same pump

• Easily selectable modes
• High Vacuum mode provides ultimate vacuum down to 1.5 x 10^-3 torr with no gas ballast
• High Throughput mode sustains long-term high inlet pressures with ultimate vacuum as low as 2.3 x 10^-2 torr without gas ballast

Adjustable gas ballast control maximizes vapor handling capability

• Gas ballast delivered into pump prevents condensation of vapor carried by pumped gases
• Three user-selectable gas ballast positions—'0' Closed, 'I' Low Flow, 'II' High Flow—meet most vapor handling requirements.

Specifications