Lonza 4D-Nucleofector Core/X unit System

The 4D-Nucleofector System was developed to offer advanced performance, flexibility and convenience for cell transfection purposes. The system has a modular architecture that allows seamless expansion of the system: it comprises a base core unit and three different functional units to suit your application of interest.

The Nucleofector System allows for transfection of difficult-to-transfect cell lines, as well as a wide variety of primary cells. Cells can be transfected in suspension, or in adherent state with the Y-unit. The electrical parameters of the Nucleofector System are optimized to gain high transfection efficiency (up to 90%) and retain high viability. Cell functionality has been shown to be conserved.

The X Unit of the 4D-Nucleofector System can handle two different Nucleocuvette Vessels both composed out of the same conductive polymer electrode material:
Single 100 μL Nucleocuvette Vessels:

• Novel conductive polymer 100 μL cuvettes replacing former aluminum cuvettes
• For high cell numbers at low throughput (e.g. for biochemical
  applications or Western Blots)
  16-well 20 μL Nucleocuvette Strips
• Same strips as those assembled to a 96-well Nucleocuvette Plate
• For low cell numbers at medium throughput (e.g. reporter gene assays, RNAi)
• Alternative version available for adherent Nucleofection

Same Conditions for Defferent Cells Numbers
As the same electrode material is now used for 20 and 100 μL cuvettes, Nucleofection Conditions are transferable between the different Nucleocuvette vessels offering maximum flexibility and convenience:

• Once the conditions are known for one format they can be easily transferred to the other format.
• Conditions are transferable between different throughput formats (4D-Nucleofector System, 96-well Shuttle Device and HT Nucleofector System).
• Existing 96-well Shuttle Protocols can be used with the 4D-Nucleofector system.

4D-Nucleofector Core Unit: the controller

Any of the units below (X, Y, LV) can be combined with the Nucleofector Core Unit. The Core Unit controls the functional units of the 4D-Nucleofector System and is equipped with:

• 5.7’’ foldable touch screen to operate the system and controlling the functional units
• Intuitive operation software for designing and saving individual experimental setups
• USB port for software update and data transfer

The X-Unit: transfection of various cell types in various formats

The 4D-Nucleofector X-Unit is the most flexible unit and supports nucleofection of various cell numbers in different formats, as it is equipped with a strip position (16-well strip format) and 2 cuvette positions. This allows you to be flexible in the transfection of your cell type of interest, using a smaller volume or a higher volume.

• Features positions for 20µl Nucleo cuvette strips (for low cell numbers down to 2 x 10^4) and 100 μl single Nucleo cuvette (for cell numbers up to 2 x 10^7)
• Different Nucleofection Vessels allow for flexible throughput from 1 to 16 samples
• Seamless transfer of conditions between different Nucleofection Vessels: same protocol can be used for use of the strips and the cuvettes.
• Comprises HV connectivity for the 96-well Shuttle Device

Overview About Nucleofection Platforms