BIO RAD QX200 AutoDG Droplet Digital PCR System

BIO RAD QX200 AutoDG Droplet Digital PCR System

**This unit has been tested to specifications. Has slight cosmetic blemishes from normal use. Software not included. Buyer can expect same unit as pictures. See photos for further details

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

• Generates droplets for 96 ddPCR reactions in under 45 minutes
• Compatible with both the QX100 and QX200 Droplet Digital PCR Systems
• A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
• Guides setup and loading with large, color touch-screen interface and LED lighting
• User-definable plate layout with increments of 8 wells provides run-to-run flexibility
• HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

• Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
• Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors
• Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

• Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. Predesigned, wet-lab validated PrimePCR ddPCR Assays are now available for mutation and copy number detection.
• Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
• Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve
• Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
• Environmental monitoring — test a wide variety of environmental samples such as soil and water
• Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

The QX200 AutoDG Droplet Digital PCR System requires the use of the PTC Tempo Deepwell Thermal Cycler or C1000 Touch Thermal Cycler with 96–Deep Well Reaction Module and the PX1 PCR Plate Sealer.

Required Consumables

• DG32 Cartridges for AutoDG
• Automated Droplet Generation Oil for Probes
• Automated Droplet Generation Oil for EvaGreen
• Pipet Tips for AutoDG
• Pipet Tip Waste Bin for AutoDG
• Droplet Reader Oil or ddPCR Droplet Reader Oil EcoTank for Probes

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

• PrimePCR ddPCR Assays
• ddPCR Supermixes for Probes (no dUTP)
• QX200 ddPCR EvaGreen Supermix
• ddPCR Supermix for Probes
• One-Step RT-ddPCR Kit for Probes

The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR) PCR System. After partitioning, the plate is sealed with the PX1 Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX600 or QX200 Droplet Reader.

• Eliminates user-to-user variability — creates more consistent droplet counts across users and plates, enabling you to fully exploit the precision of your ddPCR System
• Minimizes hands-on time — takes less than 5 minutes to load the instrument, and droplet generation requires no supervision

Features and Benefits of the Automated Droplet Generator

Bio-Rad’s Automated Droplet Generator simplifies ddPCR workflow and makes digital PCR scalable and flexible:

• Guides setup and loading with large, color touch-screen interface and LED lighting
• Generates droplets for 96 ddPCR reactions in under 45 minutes
• One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
• Provides operational flexibility:
• User-definable plate layout with increments of 8 wells
• HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
• Compatible with both QX600 and QX200 ddPCR workflows

Specifications