Miltenyibiotec -- LS Columns 25columns 130-042-401

Miltenyibiotec LS Columns 25columns 130-042-401 

**New Boxes unused, unopened. Buyer can expect same as pictures. See photos for further details.

LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells. LS Columns can be used with the following separators:

MidiMACS Separator
QuadroMACS Separator
SuperMACS II Separator, in combination with an LS Column Adapter
MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter

The matrix of the LS Columns is composed of ferromagnetic spheres, which are covered with a cell-friendly coating allowing fast and gentle separation of cells. When placed in the magnetic field of a MACS Separator, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells which are only minimally labeled with MACS MicroBeads, leaving enough epitopes free for concurrent antibody staining. The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation. LS Columns can be used to separate material less than 30 μm in size. The columns are individually packed in a sterile way.

Specifications

• Labeled capacity cells: up to 1×10/8
• Total capacity cells: up to 2×10/9
• with manual capacity use: up to 1×10/9
• with MultiMACS capacity Cell24 Separator Plus

Isolation strategy

• Positive selection
• Depletion of strongly magnetically labeled cells

Separator compatibility

• MidiMACS Separator
• Quadro MACS Separator
• SuperMACS II Separator
• MultiMACS Cell24 Separator Plus