Applied Biosystems AutoMate Xpress DNA Extraction System

Applied Biosystems AutoMate Xpress DNA Extraction System

**New Unit in box with software Included. Unopened, Unused system. See photos for further details.

The AutoMate Express Forensic DNA Extraction System is an easy to use, robust bench-top instrument that utilizes the PrepFiler Express and PrepFiler Express BTA chemistries packaged in pre-filled, foil sealed cartridges. We have designed the PrepFiler chemistry specifically to improve the quantity and quality of DNA isolated from forensic samples, thereby increasing the potential to obtain maximum information from downstream Short Tandem Repeat (STR) analysis.

Key Product Features:
• The most variable elution volume options on the market, with 7 elution volumes from 20 µL to 250 µL
• Designed to improve the yield and overall purity of DNA isolated from both routine and challenging forensic samples.
• Provides high quality DNA, free of PCR inhibitors and suitable for downstream applications such as quantitative real-time PCR and short tandem repeat (STR) analysis
• Simple and fast separation of substrate from lysate with the unique PrepFiler LySep Column
• Quick and easy run setup with ready-to-use pre-filled cartridges
• Closed system that minimizes the risk of contamination and transposition errors
• Processes 1 to 13 samples in a single run
• Rapid implementation with pre-validated, pre-programmed instrument protocols
• Integrated solution including novel reagents, consumables and automation to provide time saving, cost effective and reliable DNA extraction
• Improves downstream genotyping (STR) success rate and profile quality

PrepFiler Express Kit Format and Components
The Automate Express System utilizes two different configurations of PrepFiler Chemistry. The PrepFiler Express Forensic DNA Extraction Kit is suitable for the majority of standard sample types encountered in forensic laboratories such as bodily fluids on different substrates including FTA paper, cotton swabs, cotton cloth, denim and many others. The PrepFiler Express BTA Forensic DNA Extraction Kit was designed specifically for challenging samples such as bones, teeth and adhesive based samples such as cigarette butts and tape lifts. Both formats come with all the reagents and plastic components including the PrepFiler LySep Columns needed to perform 52 DNA extractions on the AutoMate Express Instrument.

Streamlined Off-Line Lysis
The PrepFiler Express Kits include the innovative PrepFiler LySep Column, which dramatically streamlines the off-line lysis portion of the extraction method. PrepFiler Lysis buffer or PrepFiler BTA Lysis buffer is added directly into the PrepFiler LySep Column along with the sample for incubation. Next, the PrepFiler LySep Column is quickly centrifuged which permits lysate flow through the burstable membrane while the substrate remains behind in the column. The sample tube is then simply ready to be placed on the AutoMate Express instrument. This eliminates the manual lysate and substrate transfer steps, saving time and minimizing cross contamination and sample transposition events.

The AutoMate Express Nucleic Acid Extraction System is an easy-to-use benchtop instrument that enables hands-free automation of the nucleic acid extraction process for contaminant and impurity testing. The instrument uses the established PrepSEQ nucleic acid extraction chemistry for quantitative recovery of DNA and RNA from diverse samples types, including cell culture, raw material (i.e., serum), and in-process downstream purification samples such as column purification or bulk drug substance (BDS) samples.

Features of the AutoMate Express Nucleic Acid Extraction System include:
• Time-saving, cost-effective DNA extraction for mycoplasma, MMV, and vesivirus
• Reproducible recovery of DNA from complex samples
• Out-of-the-box automation with prefilled PrepSEQ Express cartridges and ready-to-run protocols
• Closed system minimizes the risk of contamination
• Ideal for in-process testing of contaminants

Three-in-one extraction of cell culture contaminants
The AutoMate Express system can be used to perform nucleic acid extraction of the common contaminants in mammalian cell culture manufacturing, i.e., mycoplasmas, MMV, and vesiviruses (Figure 1). Flexible protocols are available to isolate DNA and RNA from a single organism, or a 3-in-1 surveillance panel protocol can simultaneously extract mycoplasma, MMV, and vesivirus nucleic acid from a single sample.

Closed system with walk-away automation
The AutoMate Express system automatically performs all steps for efficient nucleic acid extraction from contaminants in a closed environment. The system uses PrepSEQ Express kits that contain sealed cartridges with extraction reagents, and plastics, including tips and sample, lysis, and elution tubes. The workflow has been designed for a completely hands-free operation enabling reproducible extraction efficiencies and reduced time-to-results.