GE Healthcare HiLoad 26/60 Superdex 200pg chromatography column performance

GE Healthcare HiLoad 26/60 Superdex 200pg chromatography column performance

**This HiLoad Superdex is in excellent conditions and ready to be use. Looks like a new. Open Box with everything inside. See photos for further details.

Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose. Dextran component determined the separation properties of these media. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range Mr 10 000 600 000 (Superdex 200 pg).Store at +4 to +30'C. R10-Flammable, contains 20% ethanol as preservative. The most common protein purification technique that we use is gel filtration (also called size exclusion chromatography). In gel filtration, proteins are separated by their size (or more correctly by their "Stokes radius", which is largely determined by their size and shape). For gel filtration of large protein amounts, we bought GE Healthcare's HiLoad 26/60 Superdex 200 prep grade about 9 years ago (the 26/60 has been replaced by HiLoad 26/600, but both are almost identical). With this column you can very cleanly separate two proteins that have a size difference of 100%.

Therefore it is suitable to separate monomeric from dimeric versions of the same protein. The columns "expiry date" was 2012-03. Our experience is that under proper handling, such a column can easily reach a life span of 10 to 15 years (we don't use it very often, we store it in 20% ethanol, if we don't need it for longer periods of time, we keep it at +4°C). Despite its outstanding performance and without any obvious reason, we experienced a drastic reduction of separation power in the end of last year with a substantial overlap of the dimeric and monomeric peak of our pet protein VEGF-C. 

• Used for separating MAbs from dimers and contaminants
• Short run times with high recovery
• Medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions
• Separations can be scaled up to production levels
• 0.06 in. fittings to connect to AKTA design systems
• Theoretical plates: >13000m-¹
• Flow rate: <4.4mL/min.
• Flow velocity:     10m/h to 50cm/h¹
• Pressure max. (over the packed bed during operation): 3 bar [0.3MPa] (42psi)
• Media
• Average particle size: 34μm
• Matrix: composite of cross-linked agarose and dextran
• pH Stability working range: 3 to 12
• pH Stability cleaning range: 2 to 14
• Fractionation [Mr] globular proteins: 1 × 10^4-6 × 10⁵
• Fractionation [Mr] dextrans: 1 × 10^3-1 × 10⁵
• Shelf life from manufacture date: 5 years
• Chemical stability: all commonly used buffers, 1M NaOH, 0.1M HCl, 1M acetic acid, 8M urea, 6M guanidine HCl, 1% SDS, 70% ethanol, 30% iso-propanol, 30% acetonitrile

• Column
• Column I.D.: 1.02 in. (26mm)
• Inlet I.D.: 0.031 in. (0.8mm)
• Outlet I.D.: 0.031 in. (0.8mm)
• Chemical stability: resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol
• Column hardware made from borosilicate glass, polyamide, fuororubber, Ethylene Propylene Diene (EPDM), ETFE, Superpolyoxymethylene
• Column tube made from borosilicate glass
• Bed support made from Polypropylene (PP) (reinforced glass fiber), Polyamide (PA)

Specifications