Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer

Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer (with internal calibration)

**Pre-owned. Passing most positive calibrations. Full validation and calibration not included. Signal intensity is good. (Crack on left panel corner.) See photos for further details.

Includes: 

• Orbitrap Fusion Lumos
• SogeVac SV65BI FC Vacuum Pump (Qty: 2)

The Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer is the industry-leading high-performance mass spectrometer with enhanced sensitivity facilitated by a new High Capacity Transfer Tube, Electrodynamic Ion Funnel, Advanced Quadrupole Technology, Advanced Vacuum Technology, and ETD HD.

Excel at the most challenging of applications, including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, as well as MSⁿ analysis of small molecules with the new Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer. This system incorporates the brightest ion source, a segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology for improved ion transmission to the Thermo Scientific™ Orbitrap™ mass analyzer, and ETD HD, a higher-capacity ETD fragmentation. Latest innovations include the Advanced Peak Determination (APD) algorithm for improved data-dependent experiments and two new hardware options: UVPD, a new fragmentation technique achieved with a 213 nm UV laser and 1M, which provides 1,000,000 FWHM ultra-high resolution for improved structural elucidation and quantitation of isobaric compounds.

Orbitrap Fusion Lumos Tribrid MS features:

• Novel high-sensitivity API interface combines a High Capacity Transfer Tube and an Electrodynamic Ion Funnel for increased ion flux and lower limits of detection
• Advanced Active Beam Guide to prevent neutrals and high velocity clusters from entering the resolving quadrupole
• Advanced Quadrupole Technology combines high selectivity and efficiency of transfer for selected ions symmetrically across the isolation window
• Advanced Vacuum Technology improves transmission of high molecular weight ions to the Orbitrap analyzer
• Novel ETD HD—high dynamic range ETD provides significantly increased fragment ion coverage
• Advanced Peak Determination (APD) - a new algorithm for improved precursor determination in data-dependent experiments, resulting in a large increase in identification of unique peptides and experimental throughput
• Tribrid architecture includes a quadrupole mass filter, linear ion trap and an Orbitrap mass analyzer for maximal duty cycle due to a high degree of parallelization of various instrument operations
• Ultrahigh resolving power up to 500,000 FWHM, with isotopic fidelity up to 240,000 FWHM at m/z 200
• Acquisition rates of up to 20 Hz for both Orbitrap and linear ion trap MSⁿ analyses
• Full parallelization of MS and MSⁿ analyses with intelligent ADAPT™ (All Dynamically Available Parallelizable Time) technology
• Synchronous Precursor Selection (SPS) for MS and MSⁿ experiments significantly increases the number of peptides and proteins identified and improves quantitative accuracy when using isobaric mass tags
• Flexibility of fragmentation — CID, HCD and optional ETD and EThcD available at any stage of MSⁿ with detection in either the Orbitrap or linear ion trap detector enables detailed structural determination of metabolites, glycans, and other small molecules
• Universal Method provides maximal peptide identification without method optimization for samples of unknown concentration, reducing sample and instrument time requirements for routine peptide identification experiments
• Intuitive and flexible drag-and-drop user interface simplifies method development and enables unique and complex workflows
• Optional upgrades include IC, ETD, UVPD, and 1M:
  • Easy-IC - internal calibration improves mass accuracy
  • Easy-ETD - electron transfer dissociation for fragmentation of multiply charged precursors such as post-translationally modified peptides and intact proteins
  • UVPD - ultraviolet photodissociation for a wide variety of precursor ions including lipids, peptides, and intact proteins; unique to the Orbitrap Fusion Lumos Tribrid MS
  • 1M - 1,000,000 FWHM at m/z 200 ultra-high resolution improves structural elucidation and quantitation of isobaric compounds

Performance Characteristics

Mass Range
m/z 50–2,000
m/z 200–4,000
up to m/z 6,000 without precursor ion
selection (OTMS only)

Resolution OTMS
7500-500,000 (FWHM) at m/z 200

Scan Rate*
OTMSn up to 30 Hz
ITMSn up to 40 Hz

Mass Accuracy OTMS*
< 3 ppm RMS using external calibration
< 1 ppm RMS using internal calibration

MS/MS Electrospray Ionization (ESI) Ion Trap sensitivity:

2 μL of a 50 fg/μL solution of reserpine (100 femtograms total) injected at a flow
of 500 μL/min will produce a minimum signal-to-noise ratio of 200:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full scan MS/MS mode, m/z 165–615. The test requires HESI III probe.

Dynamic Range
> 5,000 within a single OTMS spectrum

MS Scan Power
MSn, for n = 1 through 10

Synchronous Precursor Isolation
Up to 20 precursors per MSn scan

Multiplexing Using the Ion Routing Multipole
Up to 10 precursors per scan using the quadrupole mass filter

Polarity Switching
One full cycle in < 1.1 sec (one full scan positive mode and one full scan negative mode at resolution setting of 30,000)

ETD Efficiency
ETD fragmentation efficiency of >15%, (infusion of 1 pmol/μL angiotensin at 3 μL/min

Analog Inputs
Channel 1 analog input (0–10 V),
Channel 2 analog (0–2 V