Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader

Absorbance photometric performance

Wavelength selection: Monochromator, tunable in 1 nm increments
Wavelength range: 200–1000 nm
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range: 0–4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0–2 OD
Photometric precision: < ±0.003 OD ±1.0%, 0–2 OD
Baseline flatness: < 0.001 OD
Stray light: < 0.05% @ 230 nm

Fluorescence photometric performance

Dual monochromators: 1 nm increment selection
EX Wavelength range: 250–850 nm
EM Wavelength range: M2: 360–850 nm, M2e: 250–850 nm
Wavelength bandwidth (EX, EM): 9 nm
Top-read optimized sensitivity: 5 pM (fluorescein) in 96 wells
Top-read guaranteed* sensitivity: 15 pM (fluorescein) in 96 wells
Bottom-read optimized sensitivity (M2e only): 10 pM (fluorescein) in 96 wells
Bottom-read guaranteed* sensitivity (M2e only): 25 pM (fluorescein) in 96 wells

Time-Resolved Fluorescence (secondary mode)

EX Wavelength range: 250–850 nm
EM Wavelength range: M2: 360–850 nm, M2e: 250–850 nm
Data collection: 50–1450 µsec., 200 µsec. increments
Guaranteed sensitivity*: 7 pM Eu-chelate

Luminescence (secondary mode)

Wavelength range (M2): 360–850 nm
Wavelength range (M2e): 250–850 nm
Guaranteed sensitivity*: 50 fM alkaline phosphatase

General photometric performance

Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier tube (PMT)
Read time**
96 wells: Abs 18 sec., FI 15 sec.
384 wells: Abs 49 sec., FI 45 sec.
Shaker time: 0 to 999 seconds
Temperature control: Ambient +4°C to 45°C
Temperature uniformity: < 1°C at 37°C set point
Temperature accuracy: ±1°C at 37°C set point