Leica TCS SP8 X w/ DMI8 Microscope Stand, Okolab Cage Incubator & Gimball Piston

Leica TCS SP8 X Confocal System w/ DMI8 Microscope Stand, Okolab Cage Incubator & Gimball Piston

***Unit was tested as far as we could. We don't have the software to fully test this system & connected devices. This unit is preowned with minimal wear from normal use. Buyer can expect everything pictured with 30-day warranty. See photos for details.

Includes:

• TCS SP8 X System
• DMi8 Microscope Stand
• Okolab Cage Incubator 
• TMC Vibration Control Cleanbench Gimball Piston 
• Picoquant PDL 800-D Pulsed Diode Laser
• SuperK Extreme EXW -12
• CTR Advanced Microscope Controller
• EL 6000 Light Source
• FLIM Pulsed Laser Interface
• Gating Box

The white light laser source of the Leica TCS SP8 X perfectly matches the wavelength of any fluorophore. Up to eight excitation lines can be used – simultaneously. By tuning both excitation and detection, complete excitation emission spectra can be acquired.

With this spectral information, any dye can be optimally excited with minimum cross-excitation and specimen damage. In addition, the pulsed White Light Laser (WLL) supplies excitation light for fluorescence lifetime measurements.

FLIM provides information about the direct environment of a molecule, like local pH or binding to another molecule.

Leica TCS SP8 X  - The Only Freely Tunable Confocal System

The white light laser source of the Leica TCS SP8 X perfectly matches the spectral properties of any fluorophore excitable in the visible spectral range. Continuously tunable excitation and detection ensure best image quality and sample protection. Up to eight excitation lines can be used – simultaneously.

› Tuning range of 470 to 670 nm in 1 nm intervals
› Minimum cross-talk in multiple stained specimen
› Change of Illumination regimes within microseconds
› Lambda Square Mapping: full spectral information by excitationemission correlation
› LightGate: background quenching using time-gated detection
› True FLIM results by adjustable excitation wavelength and variable pulse repetition rate

FREELY TUNABLE CONFOCAL IMAGING WITH THE LEICA TCS SP8 X

The White Light Laser (WLL) is the optimal excitation source to image any kind of dye combination. The acousto-optical beam splitter (AOBS) is the key to selecting any wavelength from the white spectrum. Completed by the tunable spectral detector (SP), the Leica TCS SP8 X
represents the only filter-free versatile confocal microscope.

A two-dimensional excitation-emission spectrum characterizes the spectral properties of any fluorescent specimen. Fluorophores with either identical emission spectra or excitation spectra are immediately discriminated. This is essential for optimizing multi-color fluorescence experiments.

The highest image contrast can be obtained by LightGate. This is an innovative, filter-free method to quench unwanted signals. An adjustable time gate switches off the data collection during the white light laser pulse excluding any reflected photons from the signal. Additionally, non-wanted fluorescence can be removed by adjusting the time gate.

Being a pulsed laser, the WLL is even suitable for FLIM measurements. Its continuous tunability allows you to perform FLIM measurement at any excitation wavelength. The optimal laser repetition rate is selected via an optionally integrated pulse picker, which ensures reliable FLIM results.

DMI8 

The modular DMi8 inverted microscope is the heart of the DMi8  platform solution. For routine to live cell research, the DMi8  platform is a complete solution. Whether you need to precisely follow the development of a single cell in a dish, screen through multiple assays, obtain single molecule resolution, or tease out behaviors of complex processes, a DMi8  system will enable you to see more, see faster, and find the hidden.

Flexibility is built-in, allowing you to add established options like DIC for unstained samples and Intelligent Automation. For time-lapse experiments use precise focus control with Adaptive Focus Control and Closed Loop FocusThis novel design facilitates the integration of additional fluorescence light sources and laser systems for advanced applications like:

• FRAP
• photo-switching
• ablation
• optogenetics

Every DMi8 comes standard with 19mm field of view (FOV) for all camera ports. Configure your system with a variety of contrast method, add sophisticated modalities like TIRF

SuperK Extreme EXW -12

The SuperK EXTREME supercontinuum white light lasers are broad as a lamp and bright as a laser. They deliver high brightness diffraction limited light in the entire 400-2400 nm region and by adding one of our computer controlled filters, the SuperK can be converted into an ultra-tunable laser with up to 16 simultaneous lines. Our lasers are maintenance free and the fully fiber monolithic architecture ensures excellent reliability and a lifetime of thousands of hours. The high power levels comes with an industry leading reliability of thousands of hours, and an easy to use interface making the SuperK EXTREME, simple to operate. Moreover, our unique instant-on technology and on-the-fly variable repetition rate ensures maximum productivity. The SuperK EXTREME series covers all wavelengths in the range 400-2400 nm and with more than 8 W of total power and 2W of visible power, the SuperK EXTREME lasers are the brightest supercontinuum lasers on the market.

Okolab Cage Incubator