Thermo Scientific Exactive Plus Orbitrap Mass Spectrometer

Thermo Scientific Exactive Plus Orbitrap Mass Spectrometer

 ** Unit vacuums down and shows no errors. Validation and Calibration will be needed to ensure proper functionality (not included). We can offer these services as a separate cost, inquire with us for more details. See pictures a better look at the system

The Thermo Scientific™ Exactive™ Plus benchtop LC-MS provides high-resolution, accurate-mass (HR/AM) full-scan mass spectra using proven Thermo Scientific™ Orbitrap™ technology. Advanced signal processing allows scan speeds up to 12 Hz for screening and quantitative analyses compatible with fast UHPLC chromatography. Mass resolution up to 140,000 (FWHM) at m/z 200 distinguishes target compounds from interferences, enabling analysis of demanding samples in complex matrices.

The Exactive Plus mass spectrometer is the ultimate solution for screening applications, from routine compound identification to the most challenging analysis of trace-level compounds in complex mixtures. Fast polarity switching between positive and negative scan modes saves time by allowing complete screening analyses to be completed in a single run. True high mass resolution data avoids false positives and reduces the need to reanalyze samples.

The optional HCD collision cell adds additional functionality to the benchtop system by providing all-ion fragmentation (AIF) for compound confirmation, while maintaining high-resolution, accurate-mass performance at high sensitivity.

The Exactive Plus benchtop system with full-scan and AIF modes is ideally suited for food safety and environmental analysis, forensic toxicology and clinical research, as well as drug metabolism studies. With an optional extended mass range (EMR) of up to m/z 20,000 and improved detection of high-mass ions, the Exactive Plus EMR system is an outstanding platform for biopharmaceutical and biological research requiring analysis of monoclonal antibodies, antibody drug conjugates, protein assemblies, and other proteins in their native states.

• Routine sub-ppm mass accuracy
• Resolving power of up to 140,000 (FWHM) at m/z 200
• Maximum scan speed of 12 Hz
• Intrascan dynamic range of > 5,000:1
• Mass range of 50 to 6,000 m/z (standard mode) or 350 to 20,000 m/z (EMR mode)
• Full-scan MS analysis
• All-ion fragmentation with multiple dissociation techniques:
  • - In-source CID (collision-induced dissociation)
  • - HCD (higher-energy collisional dissociation, optional)
• S-Lens for increased sensitivity
• Onsite upgradable from Exactive Plus mass spectrometer to Q Exactive mass spectrometer

Hardware Specification:

Thermo Scientific Ion Max API Source

• Enhanced sensitivity and ruggedness
• Reduced chemical noise upon sweep gas application
• 60° interchangeable ion probe orientation
• Removable metal ion transfer capillary provides vent-free maintenance
• H-ESI II probe with dual desolvation zone technology

Ion Optics

• S-Lens stacked-ring radio frequency (RF) ion guide captures and efficiently focuses the ions into a tight beam. Large variable spacing between electrodes allows for better pumping efficiency and improved
  ruggedness
• Advanced ion guides for high sensitivity and ruggedness
• High stability and ion transmission efficiency

Vacuum System

• Differentially pumped vacuum system with final vacuum <1 × 10-9 mbar
• Two split-flow turbomolecular pumps and one rotary vane pump
• Seven vacuum regions

Orbitrap Mass Analyzer

• Nitrogen- filled curved linear trap (C-Trap)
• Highly efficient ion transfer to Orbitrap analyzer
• Orbitrap analyzer with 5 kV central electrode voltage
• Low-noise image current pre-amplifier
• 16-bit signal digitalization

Data Acquisition

• Ultrafast real-time data acquisition and instrument control system
• Fully automated calibration via instrument control software
• Automated gain control

Installation Requirement:

Power

• 2 x 230 VAC ± 10% single phase, 15 Ampere, 50/60 Hz, with earth ground for
  the instrument
• 120 or 230 VAC single phase with earth ground for the data system

Gas

Nitrogen

• High purity nitrogen gas supply (99% pure at 800 ± 30 kPa (8.0 ± 0.3 bar, 116 ± 4 psi))

Environment

• System averages 2,500 W (~9,000 Btu/h) output when considering air conditioning needs
• Operating environment must be 15–26 °C (59–78 °F) and relative humidity must be 40–70% with no condensation

 Brochure