Dionex UltiMate 3000 Semi-Preparative (SemiPrep) HPLC System with VWD
- Make Thermo Scientific
- Hertz 50Hz/60Hz
- Model Dionex UltiMate 3000 Semi-Prep System
- Serial 8071560, 8075130, 8075075, 5702,1000
- Weight 240
- Voltage 120V/220V
- Working Working
- Includes ALL cables (power and data)|Computer with Software|Power Cord|User Manuals
- Warranty 30-Day Warranty, 100% Parts and Labor
- Dimensions 45×24×37 in
- Shipping Type Freight
- Item Condition Pre-owned
- Manufacturing Date 2013
Dionex Ultimate 3000 Semi-Preparative (Semi Prep) HPLC System with VWD
**This unit was tested to specifications. Buyer can expect a working system and 30-day warranty. Unit has slight wear from normal use. Everything pictured included. See photos for further details.
Includes:
- VWD-3100 Variable Wavelength Detector P/N 5074.0005
- TCC-3000SD Thermos tatted Column Compartment P/N 5730.0010
- HPG-3200BX Biocompatible Binary Semi-Preparative Pump P/N 5042.0025
- AFC-3000 Automated Fraction Collector w/ racks (x2) P/N 5702.1000
- Dell OptiPlex 9010 with Windows 7 pro & Chrome Leon 6.80 installed.
- Dell Monitor & Backups Pro 1500 UPS
The Thermo Scientific Ultimate 3000 Binary Semi-preparative system is a workhorse that will always be ready to assist with your purification of natural product extracts, isolation of impurities, or other large-scale LC processes.
The Ultimate 3000 Binary Semi-preparative system is tailored for robust, safe, and high-performance purifications. Based on proven high-pressure gradient principles, the system takes full advantage of Ultimate 3000 features such as Smart Flow pulse-free eluent delivery and wide linear range detection.
VWD-3100 Variable Wavelength Detector P/N 5074.0005
The increased sensitivity of variable wavelength detectors makes them an excellent choice for applications involving trace analytes. Their wide dynamic range also makes them especially suited for pharmaceutical applications involving multiple compounds at very different concentrations.
The four-channel Ultimate 3000 RS Variable Wavelength Detector VWD-3400RS is ideal for research and more complex methods involving analytes with different absorption maxima. For conventional LC applications, this detector allows data acquisition from up to four UV-vis channels at up to 5Hz (up to 200Hz in single-channel mode).
The Ultimate 3000 Single Channel Variable Wavelength Detector VWD-3100 is ideal for routine methods such as quality control applications employing isocratic separations.
Features
- Low noise (typically < ±2.5 µAU) and drift (< 0.1 Mau/h) ensure high sensitivity.
- Linear range of up to 2.5 AU allows for the analysis of a wide range of analyte concentrations.
- Easily adapts for applications from nano preparative through to semi-preparative HPLC.
- Data collection rates of up to 200Hz ensure detection of even the sharpest peaks.
- Predictive performance technology continually monitors system performance to warn of impending errors, such as lamp failure.
- Spectrum scanning functionality enables analyte characterization.
Monitor Gradients Online
The Thermo Scientific™ Dionex™ Ultimate™ 3000 PCM-3000 pH and Conductivity Monitor is an upgrade for accurate pH and conductivity on-line monitoring with the VWD-3000 Series. This unit is typically used to monitor the gradient formation in salt- or pH-gradient-based ion-exchange chromatography (IEC) in biopharmaceutical analysis.
The PCM-3000 supports all modes of chromatography and provides verification of the elution conditions, the retention behavior of the sample, and the precision and repeatability of the instrumentation.
TCC-3000SD Thermos tatted Column Compartment P/N 5730.0010
Features
- Temperature range of 5° to 80°C ±0.5°C and stability of ±0.1°C simplifies method optimization.
- Precise temperature control under varying ambient conditions ensures retention time precision.
- Fast heat-up/cool down times minimize delays when changing between methods.
- Eluent preheaters deliver sharper peaks and better resolution.
HPG-3200BX Biocompatible Binary Semi-Preparative Pump P/N 5042.0025
Purify biological compounds in the microgram to lower-milligram range quickly and accurately with the Thermo Scientific™ Ultimate™ HPG-3200BX Biocompatible Binary Semi-Preparative Pump. Binary high-pressure proportioning offers gradient accuracy and precision. Maximize your flow rate depending on your application. This pump supports operating pressures of up to 16.5 MPa (2400 psi/165 bar) at up to 30mL/min.; 13.5 MPa (1950 psi/135 bar) at 50mL/min. For isocratic applications, Double Flow mode enables flow rates of up to 100mL/min.
The HPG-3200BX pump is the right choice for laboratories that need to purify compounds in the microgram to lower-milligram range. It is recommended for semipreparative separations on 4.6–21.2 mm id. columns.
Features- Serial dual-piston operating principle for outstanding flow rate accuracy and precision across the entire flow rate range
- Wide operating flow rate range from 0.5 mL/min to 50 mL/min.
- Support operating pressures of up to 165 bar (2400 psi) at up to 30 mL/min; 135 bar (1950 psi) at 50 mL/min
- Double Flow mode enables flow rates of up to 100 mL/min for isocratic applications.
- Binary high-pressure proportioning for best-in-class gradient accuracy and precision
- Smart Flow™ technology automatically ensures optimal chromatographic performance at any operation condition.
- Automatic compressibility compensation, no manual solvent setting needed.
- Active rear-seal wash and floating pistons for maximum seal lifetime
AFC-3000 Automated Fraction Collector w/ racks (x2) P/N 5702.1000
The Thermo Scientific™ Dionex™ AFC-3000 Automated Fraction Collector is a rugged and flexible fraction collector, ideal for preparative reversed-phase or normal phase HPLC as well as purification of proteins, peptides, and nucleotides. The AFC-3000 meets the small peak volume requirements of UHPLC applications and manages analytical and semipreparative to preparative flow rates. It collects fractions into an industry leading variety of vessels, such as 96 well plates, standard vial and tube sizes, and bottles. A funnel rack directs virtually.
unlimited volumes into any bottle or reactor.
Features
- Rugged and easy-to-use design
- Very fast tube changes for ultrafast purification
- Flow rates up to 150 mL/min
- Optional kit for small-peak-volume compatibility and improved performance at low flow rates
- Smallest possible carryover through minimized delay volume between valve and drop former.
- Large variety of collection vessels
- Large capacity: holds up to 180 tubes or 4 × 96 well plates.
- Resistant against a broad range of solvents, buffers, acids, and bases
- Optional grounded drop former for safe operation with normal phase eluents
- Ideally suited for working with sophisticated Thermo Scientific™ Dionex™ Chrome Leon™ software fraction collection features
Manufacturer's Specifications:
- VWD-3100 Variable Wavelength Detector P/N 5074.0005
| Analog Outputs | Up to 2 Channels (Optional) |
| Automated Start-Up Functions | Wavelength Verification |
| Conductivity Wetted Parts | PEEK, Quartz Glass, Stainless Steel |
| Description | VWD-3100 Variable Wavelength Detector, one channel, without flow cell |
| Data Collection Rate | 100 Hz |
| Detection Mode | UV-vis Detection (Single Channel) |
| Detector Type | Variable Wavelength |
| Type | Variable Wavelength Detector |
| Display | Front Panel |
| Drift | <1 x 10-44 AU/hr. |
| Filter | Fast Switching Second Order |
| Lamp | Deuterium Lamp, Tungsten Lamp |
| Linearity | <5%RSD |
| Noise | <±3.5 µAU |
| Optical Bandwidth | 6 nm |
| pH Wetted Parts | PEEK, Quartz Glass, Stainless Steel |
| Safety Features | Power-up Diagnostics of Optics, Cooling Fans, Motors, and Electronics Leak Sensor |
| Temperature Control | Yes |
| Wavelength Accuracy | ±1.0 nm |
| Wavelength Range | 190 to 900 nm |
| Voltage | 85 to 260 V |
| Unit Size | Each |
- TCC-3000SD Thermos tatted Column Compartment P/N 5730.0010
| Description | TCC-3000 SD Thermos tatted Column Compartment |
| For Use With (Equipment) | Ultimate 3000 SD Series |
| Type | Standard Thermos tatted Column Compartment |
| Voltage | 150 V |
| Recommended Eluent Preheater Volume | 2 μL |
| GLP Features | Dionex AutoQ routines for Instrument Qualification Column Identification System for four columns. |
| I/O Interfaces | 2 digital inputs, 2 programmable relay outputs |
| Cool-Down Time | 15 min. |
| Max. Column Length | 30 cm |
| Heat Up Time | 15 min. |
| Column Capacity | Up to 12 columns, depending on column length; Max. column length: 30 cm. |
| Max. No. of Switching Valves | Up to 2 Valves: 2-position/6- or 10-port, 6-position/7-port |
| PC Connection | USB |
| Temperature Accuracy | ±0.5°C |
| Temperature Range | 5°C to 80°C |
| Weight (Metric) | 12.6 kg |
| Unit Size | Each |
- HPG-3200BX Biocompatible Binary Semi-Preparative Pump P/N 5042.0025
| Flow Accuracy | ±0.1% at 3 mL/min typically ±0.1% (at 25 mL/min) |
| Flow Precision | <0.05% RSD or <0.01 min SD, whichever is greater. |
| Gradient Formation | High-pressure proportioning |
| Max. Flow Rate | 50.0 mL/min. |
| Pressure Range | 2 to 16.5 MPa up to 30 mL/min; 2 to 13.5 MPa up to 50 mL/min |
| GLP Features | Full support of Thermo Scientific Dionex AutoQ instrument qualification, qualification status and system wellness monitoring. All system parameters are logged in the Chrome Leon Audit Trail. |
| Gradient Delay Volume | 800-1550 µL Default: 800 µL |
| I/O Interfaces | 2 digital inputs, 2 relay outputs. |
| Min. Flow Rate | 0.001 mL/min. |
| No. of Eluent Lines | 2 |
| Operating Principle | Serial dual piston |
| Pressure Ripple | Typically: <0.2 MPa or <1% whichever is greater. |
| Proportioning Accuracy | ±0.2% (full scale) |
| Proportioning Precision | < 0.2% SD |
| Unit Size |
Each |
- AFC-3000 Automated Fraction Collector w/ racks (x2) P/N 5702.1000
| Tube capacity: | Depends on rack configuration. standard configuration: 180 x 8 mL tubes (13 mm x 100 mm) |
| Tube change time: | Typically, 0.2 to 0.4 s, depending on collect mode and rack type. |
| Maximum tube height: | 150 mm |
| Maximum flow rate: | 150 mL/min |
| Carry-over volume: | 15 µL (standard), 1.3 µL (with optional low-flow kit) |
| Wetted parts: | ETFE, FFKM (Simrin®), PFA, PEEK (with standard and low flow drop former), stainless steel (with grounded drop former for normal-phase LC) |
| Control: | All functions software-controlled via RS-232 or USB; requires Chrome Leon Chromatography Management System |
| GLP features: | In Chrome Leon: All system parameters are recorded in the Chrome Leon audit trail. |
| Power requirements: | 100–240 V, 47-63 Hz AC 1.9 A with an output of DC 24V, maximum 3.33 An Automatic voltage selection |
| Emission sound pressure level: | < 70 dB(A), typically 62 dB(A) |
| Environmental conditions: |
Range of use: Indoor use Temperature: 10 to 35 °C (50 to 95 °F) Air humidity: 20-70% relative humidity, non-condensing Overvoltage category: II Pollution degree: 2 |
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