Bio Rad Protean i12 IEF Cell Isoelectric Focusing CLEARANCE! As-Is

Bio Rad Protean i12 IEF Cell Isoelectric Focusing Protein System

 ***This instrument has been tested to factory specifications. Buyer can expect a working machine and 30-day warranty. Unit has slight wear from normal use.  See photos for details.

The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.

Features and Benefits 

• Optimize experiments in fewer runs — the PROTEAN i12 IEF system has the unique ability to run channels under multiple conditions in a single run. Different samples, protocols, and pH gradients can be run simultaneously.
• Obtain higher-quality data with less experimental risk — each channel is independent, so one irregular sample can no longer compromise the entire run.
• Easy, intuitive programming — easily run, create, and edit isoelectric focusing protocols from the touch screen user interface. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized sample specific IEF conditions.
• Run gel side up, gel side down, or with loading cups — focusing trays and electrode assemblies accommodate all possible gel configurations.

Applications and Uses 

Isoelectric focusing is the first dimension of 2-D electrophoresis, a powerful technique used for the visualization and identification of proteins. The PROTEAN i12 IEF system can be used for a number of applications including the following:

• 2-D electrophoresis and analysis
• Protein profiling — analysis of complex protein mixtures
• Biomarker discovery — discovery/identification of specific targets and markers
• Functional proteomics — structure, interactions, and function

Manufacturer's Specifications:

• Input power 100–240 VAC, 50/60 Hz
• Fuse Two 6.3-amp, time delay, 5 × 20 mm
• Power input IEC 60320 standard cord set with ground.
• Power output
  
  • Voltage per lane 0, 50 –10,000 V
  • Current per lane 0–100 μA, 1 μA intervals
  • Power 0 –1 W per lane
• Peltier platform
  
  • Tray capacity 1 tray
  • Temperature 10–25ºC ± 0.5ºC @ max. ambient temperature 23ºC
  • 15–25ºC ± 0.5ºC @ max. ambient temperature 31ºC
• Focusing trays
  
  • Material Polycarbonate
  • IPG strip length 7, 11, 13, 17, 18, and 24 cm
  • Capacity 1–12 IPG strips per focusing tray.
  • Maximum 7 cm: 7 ml; 11 cm: 10 ml; 13 cm: 11.2 ml.
  • channel volume 17 cm: 14.2 ml; 18 cm: 15.2 ml.
  • 24 cm: 20.2 ml
• Rehydration/equilibration trays
  
  • Material Polystyrene
  • IPG strip length 7, 11, 13, 17, 18, and 24 cm
  • Capacity 1–12 IPG strips per tray
  • Maximum 7 cm: 6.8 ml; 11 cm: 9.6 ml; 13 cm: 10.5 ml.
  • channel volume 17 cm: 14.2 ml: 18 cm: 16 ml; 24 cm: 19 ml
• Environmental requirements for indoor use only, at altitudes of up to 1829 m (6000 ft). Operates at 10–35ºC ambient temperature, with maximum 90% relative humidity.
• User Interface
  
  • Display QVGA resolution (320 × 240)
  • Touch screen or mouse control
  • Programmable Yes
• Ramping
  • Step, linear, gradual, and hold voltage.
    ramping used for each focusing step.
    Hold mode as a final step to prevent.
    diffusion when focusing is complete.
• Protocol capacity 2 GB, storage of approximately 20,000 data and protocol files
• Data collection .DAT file format