ProteinSimple Peggy Sue Automated Western Blot CLEARANCE! As-Is
- Make Proteinsimple
- Hertz 50Hz/60Hz
- Model Peggy Sue
- Serial AB-0528 & SW-1559
- Weight 290
- Voltage 120V/220V
- Working Not Working
- Includes ALL cables (power and data)|Power Cord
- Warranty AS-IS
- Dimensions 28×43×41 in
- Shipping Type Freight
- Item Condition For parts or not working
ProteinSimple Peggy Sue Automated Western Blot System with Auxilary Module - For Parts
**This instrument is for sale AS/IS. Unit powers on with Red error light. Slight wear from normal use around unit. Everything pictured is included. See photos for further details.
- Size and charge-based automated capillary Western blot assays
- Up to 96 samples
- Chemiluminescence
A Simple Western assay is an automated Western - no gels, no transfer devices, no blots, no film and no manual analysis. Simple Westerns take place in a capillary. Peggy Sue can automate all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 12 samples can be analyzed simultaneously in a single cycle and up to 8 cycles (96 samples) can be processed in a single run. Peggy Sue can do both size-based and charge-based assays. All you have to do is pipette your samples, separation reagents and immunoreagents into the plate, load the capillaries and press start.
How Can Peggy Sue Help You?
My sample is precious and I don't have enough for other methods
Sometimes you wish you could do more with your small and precious sample. Peggy Sue, our most frugal system can run up to 96 data points in one overnight run with as little as 5 µL of sample. And she can utilize that sample to the max by allowing you to interrogate those few microliters eight times, such as when analyzing an entire cell signaling pathway in a single run. Imagine all the data you can get from your sample!
Applications:
- Cancer & Immuno-Oncology - Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.
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SARS-CoV-2 - Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!
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Cell & Gene Therapy - Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
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Targeted Degradation - Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.
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SARS-CoV-2 Serology - Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!
- Exosome - A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.
Auxilary Module: The auxiliary module holds the water and waste bottles used by Sally Sue and Peggy Sue. The waste bottle has a yellow label, and the water bottle has a blue label. The volume in each reservoir is 1.0 L. Deionized water is used by the instrument to clean the troughs in the separation tray and the fluidics in the
head assembly. The waste bottle collects waste generated during the run. There are two liquid level sensors in each bottle. The sensor at the bottom of the bottle detects when the bottle is empty, and the sensor at the top of the bottle determines if sufficient volume is available to complete an eight-cycle assay with the instrument.
Manufacturers Specifications:
| Description | Total Protein Specification | Immunoassay Size Specification | Immunoassay Charge Specification |
|---|---|---|---|
| Sample Required | 0.3-1.2 µg lysate | 0.6-1.2 µg | 0.6-1.2 µg |
| Volume Required | 3-5 µL/well | 3-5 µL/well | 5-12 µL/well |
| Size or pI Range | Molecular weight (MW) ladders range from 2-440 kDa | Molecular weight (MW) ladders range from 2-440 kDa | Widest gradient ranges from pI 3 to pI 10 |
| Sizing CV | <10% | <10% | <10% |
| Intra-assay CV | <15% | <15% | <20% |
| Inter-assay CV | <20% | <20% | <20% |
| Resolution (± percent difference in MW) | ± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa | ± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa | ± 0.1 pI units |
| Quantitation CV | <20% | <20% | <20% |
| Dynamic Range | 2-3 logs | 3 logs | 3 logs |
| Sensitivity | ng | Low pg | Low pg |
| Capillary | 5 cm, 100 µm, 400 nL | ||
Testimonials
“REUZEit has been a great partner for our used equipment needs and always provide timely updates of new arrivals of consigned equipment.”
“Great company to work with. Tammy completed our first international equipment shipment with ease.”
“Fast response, open to adjusting schedule as needed, and great customer interaction.”
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