Biotek nergy Microplate-Detection for HTS and CLEARANCE! As-Is

NOTE: Unit sold AS-IS, needs repairs, and does not include computer/monitor/software (even though shown in images).

Biotek Synergy 2 Microplate-Detection 

**Unit has been tested in diagnostics and ran with the computer software. Unit needs repairs. Black computer pictured is not included. Images of results are included. All buttons and switches are working fine. Unit has a few signs of cosmetic wear... see photos for more. This unit needs repairs and is sold as-is.

Background/Description 

The BioTek Synergy 2 is an easy-to-operate multimode microplate reader built for drug discovery (R&D) and the biotech life science research arena. This modular system is currently configured with Absorbance, Luminescence, and Fluorescence capabilities. Luminescence is measured with a low noise PMT, through an empty filter position in the Emission filter wheel. Filters can also be used if light filtering is necessary.

Synergy 2 has monochromator-based UV Vis Absorbance, top and bottom fluorescence intensity, and a Time-Resolved Fluor Module as well. In addition, this Biotek microplate reader has 4 zone temperature control, with a range of between 4-50°C, as well as three speeds of shaking. The Synergy 2 Luminescence Plate reader will accommodate standard 6-, 12-, 24-, 48-, 96-, and 384–well microplates as well as the BioTek Take3 Multi-Volume Plate. This model is compatible with the Dual Reagent Dispenser Module, however, that is sold separately.

The Synergy 2 Plate Reader is controlled by a computer using BioTek’s Gen 5 Software which is compatible with Alpha Screen assays and certified for HTRF, DLR, and Lanta Screen. This Biotek microplate reader is robot accessible and compatible with BioTek’s BioStack Microplate Stacker. Gen5 supports OLE automation to facilitate Synergy 2’s integration into an automated system.

Information from Research Study 

The Synergy™ 2 is a new type of reader that provides research
laboratories performances usually found on high-end HTS instrumentation, while delivering flexibility and efficient cost-control to screening laboratories. The Synergy 2 utilizes multiple optics to provide performance regardless of the detection technology. Absorbance measurements use a xenon lamp with a monochromator for wavelength selection from 200 nm to 999 nm. Fluorescence measurements are made using either a tungsten halogen or a xenon-flash lamp with filters and dichroic mirrors and a PMT for detection. Fluorescence polarization is accomplished with polarizing filters in conjunction with dichroic mirrors. For time-resolved fluorescence measurements, the Synergy 2 integrates a high-energy xenon-flash lamp with excitation and emission filters and PMT detector. Luminescence measurements are accomplished by using a liquid-filled light guide. The Synergy 2 is capable of reading any plate format up to 1536 wells, is robotic compatible and provides temperature control and shaking. Reagent injectors are also available.

Multi-mode microplate readers are designed either for screening
or for research applications. In the first instance, they are fast endpoint readers that use homogeneous detection technologies. Readers designed for research applications are more flexible but with lower levels of performance. The Synergy 2 is a new type of reader that provides the combined benefit of bringing to research laboratories performances and technologies usually found on high-end HTS instrumentation, while at the same time delivering flexibility and efficient cost-control to screening laboratories. The Synergy 2 utilizes multiple sets of optics to provide optimal performance regardless of the detection technology. Absorbance measurements use a xenon-flash lamp with a monochromator for wavelength selection, allowing the selection of any wavelength for endpoint or kinetic measures from 200 nm to 999 nm. Fluorescence measurements are made using either a tungsten-halogen lamp or a xenon-flash lamp with bandpass filters with or without dichroic mirrors for wavelength selection and PMT for detection. As little as 1 PM fluorescein can reliably be detected. Fluorescence polarization is accomplished with the use of polarizing filters in conjunction with label specific dichroic mirrors for wavelength specificity. For time-resolved fluorescence measurements, the Synergy 2 integrates a high-energy xenon flash lamp with excitation and emission filters and PMT detector. Concentrations of europium as low as 60 FM can be detected.

Luminescence measurements are made using a liquid-filled light
guide to capture light along with a low noise PMT. Detection limit of 30 a mole of ATP has been observed. The Synergy 2 is capable of reading plate formats up to 1536 wells, is robotic compatible, provides temperature control, and includes shaking as standard features. Examples of typical performance of the instrument, including fluorescence polarization; DLR luminescence; LANCE, and DELFIA time-resolved fluorescence assays will be provided, along with an overview of the system.