GE AKTApilot Liquid Chromatography System (LC) 56317181

GE AKTApilot Liquid Chromatography System (LC) 56317181

ÄKTApilot™ is a high performance, automated liquid chromatography system.
The system is designed for process development, process scale-up and scaledown, and small-scale production.

ÄKTApilot is biocompatible, hygienic and subitizable. It meets all GLP and cGMP
demands for Phase I–III in drug development and small-scale production.

ÄKTApilot system consists of ÄKTApilot separation unit, the connected computer
including a flat-screen monitor, and UNICORN™ control system.

•  Liquid flow rates from 4 to 800 ml/min (limited gradient performance at 400–800 ml/min).
• Operating pressure up to 20 bar.

Principle of ÄKTApilot
ÄKTApilot contains all the fluid handling components required to perform buffer
delivery and mixing, sample application, and in-line monitoring.

Detectors and monitors
ÄKTApilot is equipped with detectors for continuous in-line measurement of pH,
conductivity and UV absorbance. The detectors provide accurate and reliable
monitoring through self-test and self-calibration.  The flow cells are placed close together and are easily accessible from the front panel to facilitate maintenance. As a result, band broadening and time delay between detectors are lessened.

Pre-programmed method templates and optimized system configurations minimize preparation time by allowing rapid media screening, method scouting and optimization as well as scale up experiments. Dedicated method templates are supplied for the most common scouting procedures including automatic media screening and automatic eluent pH scouting for ion exchange chromatography. Convenient sample application and column packing is also possible by regulating the flow rate, while pressure can also be monitored through four points.

Isocratic and gradient flow rate4–400 mL/minDouble mode flow rateUp to 800 mL/minOperating pressureUp to 2 MPa (20 bar, 290 psi) between system pump and column inletUV wavelength range190–700 nm in steps of 1 nm, 206–700 nm with AKTApilot cell, 3 wavelengths simultaneouslypH range0–14 (spec. valid between 2–12)Conductivity range1 µS/cm to 999.9 mS/cmHoldup volumeUp to 31 mlMax backpressureless than 0.06 MPa before column/ 400 ml/min less than 0.045 MPa after column /400 ml/minSolvent compatibilityAll commonly used chromatographic solventsDegree of protectionIP 24