Roche LightCycler 480 II /96 Real Time PCR System

Roche LightCycler 480 II /96 Real Time PCR System

Rapid high-throughput, plate-based real-time PCR amplification and detection instrument. Combining amazing speed with exceptional accuracy, the LightCycler® 480 Real-Time PCR System is a novel platform for gene-expression and melting curve-based mutation analysis.

The compact yet versatile LightCycler® 480 benchtop instrument can be interfaced with robotics to create an automated high-throughput solution. Based on innovative hardware and software, the LightCycler® 480 Instrument is a modular online PCR device for qualitative or quantitative detection of nucleic acids, genotyping, and mutation analysis.

It will meet the needs of a broad range of scientific applications in genomics research, such as array validation, gene-knockdown studies, or SNP analysis. Offering the sensitivity and accuracy one has come to expect only from the Roche Applied Science LightCycler® System, the LightCycler® 480 Real-Time
PCR Instrument goes one step further in providing enhanced throughput and multiplexing capabilities. 

Supporting all current probe formats and applications in gene-expression and mutation analysis, its built-in versatility allows adaptation to future technological advances in genomic research.

• Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis. Use basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves.
• Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity.
• Use a wide range of probe formats (e.g., FRET or HybProbe probes, SimpleProbe probes, hydrolysis probes) and fluorescent dyes (e.g., SYBR Green I, ResoLight Dye, FAM, HEX, Cy5, LightCycler^® Red 610, LightCycler^® Red 640, and LightCycler^® CYAN 500).
• Choose the optimal type of multiwell plate (white or clear) to best fit your assay format, application, and workflow. Use the available adapter plate to also work with LightCycler^® tube strips.
• Build a modular real-time PCR platform to meet your particular needs. Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional user-friendly software modules.
• Extract meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines. Analyze true raw data without the need for passive reference dyes or normalization plates.
• Protect your data using a system compatible with 21 CFR part 11 requirements.
• Integrate the LightCycler^® 480 System as part of a LIMS in combination with robotic pipetting and plate loading.

**This instrument is for sale AS/IS due to faulty lamp module. Unit has some slight discolorations on front and some scratches on side panel from normal use.  Everything pictured included. See photos for details. 

Temperature control: 

• Peltier-based heating/cooling from 40°C - 95°C
• Heated lid, PCR without any overlay (e.g., wax or oil)
• Passive post-run cooling to < 40°C

Excitation: 5 excitation filters, high-intensity broad-spectrum xenon lamp

Detection: 6 detection filters, signals detected online by CCD camera

Run time: < 60 minutes (96-well plate)
< 40 minutes (384-well plate) 

Dynamic range: 1 to 108 copies/well detectable (viral target)