Perkin Elmer VICTOR 3V 1420 Multilabel Plate Counter

VICTOR3V is a multilabel, multitask plate reader for all light-emitting and light-absorbing detection technologies including: fluorescence (top and bottom), luminescence, absorbance, UV absorbance, time-resolved fluorometry, and fluorescence polarization.

Manufacture Specification 

Fluorometry

• Fluorescein < 2 fmol/well • Linearity > 5 decades

• Crosstalk < 0.0001

• Umbelliferone < 200 fmol/well

• Rhodamine < 100 fmol/well

TR-fluorometry

• Eu typically < 10 amol/well

• Eu linearity > 5 decades

• Crosstalk < 0.0001

• Tb typically < 5 amol/well

• Sm typically < 50 amol/well

• Dy typically < 150 amol/well

Photometry

• Measuring range @ 405 nm 0 – 2 A

• Accuracy @ 405 nm < 2% or (0.01A)

• Precision @ 405 nm < 0.5% or (0.01A)

Luminometry

• Lower limit of detection < total flux of 105 photons/sec

• ALP typically 1 amol/well with AMPPD substrate

• ATP typically 80 amol/well in flash assays

• Luciferase 0.9 pg/well using Genglow-100 or GeneLux

Fluorescence Polarization

• Fluorescein 1 nM (384-well plate)

• Standard deviation < 5 mP