Applied Biosystems 7500 Fast Real time PCR Testing System- Ref 4357362

Applied Biosystems 7500 Real time PCR Testing System

** This unit has been tested and is working to specifications. Buyer can expect a working unit and it comes with a 30 day warranty. Unit has slight signs of use, but overall looks in very good condition.

The Applied Biosystems 7500/7500 Fast Real-Time PCR System uses
fluorescence-based polymerase chain reaction (PCR) reagents to provide:
• Quantitative detection of target nucleic acid sequences (targets) using real-time
analysis.
• Qualitative detection of targets using post-PCR (endpoint) analysis.
• Qualitative analysis of the PCR product (achieved by melt-curve analysis that occurs
post-PCR).

About Data Collection: 
The 7500/7500 Fast system collects raw fluorescence data at different points during a PCR, depending on the type of run that the instrument performs:

Real-Time runs: Standard curve, Relative standard curve, Comparative CT 

The instrument collects data following each extension
step of the PCR.

Post-PCR(endpoint)runs: Genotyping and Presence/Absence

The instrument collects data:
• Before the PCR (For presence/absence experiments,
data collection before the PCR is optional but
recommended.)
• (Optional) During the PCR. The instrument can collect
data during the run (real-time); collecting data during
the run can be helpful for troubleshooting.
• After the PCR.

Regardless of the run type, a data collection point or read on the 7500/7500 Fast instrument consists of three phases:

1. Excitation – The instrument illuminates all wells of the reaction plate, exciting the
fluorophores in each reaction.
2. Emission – The instrument optics collect the residual fluorescence emitted from the
wells of the reaction plate. The resulting image consists only of light that
corresponds to the range of emission wavelengths.
3. Collection – The instrument assembles a digital representation of the residual fluorescence collected over a fixed time interval, then stores the raw fluorescence image for analysis.
After a run, the 7500 software uses regions of interest (ROI), optical, dye, and background calibration data to determine the location and intensity of the fluorescence signals in each read, the dye associated with each fluorescence signal, and the significance of the signal.