Waters HPLC 1525 Binary Pump System with 2487 Dual Absorbance Detector

Control

MassLynx 4.0(SP1) and Breeze 3.3 software

Programmable flow rate

0.00 to 5.00 mL/min, in 0.01-mL/min increments

Flow precision

2 secs, SD, replicates based on retention time or  volumetric measure at 0.2 mL/min 1000-2000 psi backpressure, PQ test (each pump individually)1

Flow accuracy

2% of setting at 0.1mL/min using degassed methanol at 1000-2000 psi backpressure (each pump individually)

Maximum operating pressure

6000 psi (41,370 kPa, 400 bars)

Operating pressure limits

Software programmable high and low pressure limits; user selectable in psi, kPa,bar

Delay volume

<30 µL (without mixer) , 100 (with one 50 µL mixer)

Gradient accuracy

<1% of setting (typical) between 10 to 90%, and 1000 psi backpressure (methanol: methanol with propylparaben and one 50 µL mixer)

Gradient Precision

<0.5% RSD (typical) between 10 to 90%, 0.1 mL/min, and 1000 psi backpressure (methanol: water  with octanophenone and one 50 µL mixer)

Pressure Ripple (one pump)

<2.0% at 0.5 mL/min, degassed methanol, at 1000 to 2000 psi backpressure

Gradient Ripple

<1.5% normalized to full scale between 10 to 90%, 0.1 mL/min, and 1000 psi backpressure (methanol: methanol with propylparaben and one 50 µL mixer)

Wavelength Range

190 to 700 nm

Bandwidth

5 nm

Wavelength Accuracy

± 1 nm

Wavelength Repeatability

± .10 nm

Noise, single wavelength

< ± 0.35 x 10-5 AU, dry cell, 254 nm, 1.0 sec. time constant (TC)

Noise, dual wavelength

< ± 2.5 x 10-5 AU, dry cell, 254 nm / 280 nm, 2.0 sec. time constant (TC)

Linearity

5% at 2.5 AU, dry cell, 254 nm / 280 nm, 2.0 sec. time constant (TC)

Drift

1.0 x 10^4 AU/Hour/degrees Celcius at 254 nm (after 1 hour warmup)

Sensitivity setting range

0.0001 to 4.0000 AUFS