Description
The Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.
• 96-well format eases plate setup if automation is not available
• Accommodates tube strips, which can be capped immediately after pipetting each sample
• Fast mode completes runs in less than forty minutes
• Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
• 5-color variable excitation enables multiplex assays
Manufacture Specification
| Format: | 8-well strips, 96-well plate |
|---|---|
| Optics: | Halogen light source, Five excitation and five emission filters, CCD Camera |
| Capacity: | 1 x 96-well plate (Fast), 12 x 8-tube strips |
| Precision: | Distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence |
| Dimensions: | 34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D) |
| Throughput: | 4 to 5 96-well plates per day |
| Sensitivity: | Down to 1 copy of human RNAse P gene |
| Product Size: | 1 system |
| Dynamic Range: | 9 logs of linear dynamic range |
| Calibrated Dye: | TAMRA, JOE, Cy5, Texas Red, NED, ROX, VIC, Cy3, FAM, SYBR Green |
| Reaction Speed: | Standard, Fast |
| Sample Ramp Rate: | Standard mode: ± 1.6°C⁄sec, Fast mode: ± 3.5°C⁄sec |
| External Computer: | Computer tower provided |
| Peak Block Ramp Rate: | 5.5°C⁄sec |
| Temperature Accuracy: | ±0.5°C |
| Passive Reference Dye: | ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed) |
| Reaction Volume Range: | 10-30 µl |
| Temperature Uniformity: | ±1°C |
|
Thermal Cycling System: |
Peltier-Based System |
|
Temperature Range (Metric): |
4.0-99.9 °C |
