Applied Biosystems MDS SCIEX Tempo Nano Autosampler

Key Advantages of the Tempo™ Nano LC Systems

• Direct pumping system and the measurement of flows with active feedback ensures precise gradient delivery, providing excellent run-to-run reproducibility
• Optimized for low flow rates between 20–1000 nL/minute, (and 1-20 µL/min for the first dimension pump in MDLC system)
• Ultra low delay volume of 65 nL from mixer to injection valve enables rapid gradients and re-equilibration to shorten total cycle times
• Elimination of flow splitting reduces operating costs due to low solvent consumption and minimal waste production
• Full MDLC functionality available
• Easy-to-use software interface
• Simplified plumbing scheme and splitless design make for a reproducible, easy-to-use system

Retention Time ReproducibilityMany LCMS applications, such as biomarker discovery by MS profiling, metabolomics, peptide quantitation, etc., require the comparison of chromatographic peaks across many replicate injections. Quantitative peptide/protein analysis in complex mixtures is typically done using multiple reaction monitoring (MRM) on a 4000 Q TRAP® system and requires a high degree of reproducibility across many injections. For this type of quantitative work, the peak area of a peptide at a specific retention time from an unknown sample is compared to a standard concentration curve of a reference and the unknown concentration is calculated.