Invitrogen ZOOM IEF Fractionator

The ZOOM IEF Fractionator enriches low abundance proteins and increases the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two-dimensional gel electrophoresis (2DE), one-dimensional gel electrophoresis (1DE), or two-dimensional liquid chromatography/mass spectrometry (2D LC/MS).

• Built for safety, reliability, and durability
• Includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid
• Use with ZOOM Disks and ZOOM Reagents

• Sample Chamber and Fractionator Unit Set-up Procedure

  • The sample chamber and components fit together easily and offer a leak-proof seal
  • Simply place disks between the sample chambers in the ZOOM IEF Fractionator to allow separation in a specific pH range; for example, to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM Disk of pH 4.6 and one of pH 5.4
  Downstream Processing by 2D Gel Electrophoresis
  • Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM Strips on the ZOOM IPGRunner System
  • Follow with second dimension analysis using neutral pH NuPAGE ZOOM Gels

   

  Applications:

  Isoelectric Focusing, Protein Gel Electrophoresis, Protein Sample Fractionation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis