The Mastercycler gradient sets the standard for optimization of PCR reaction conditions. A freely programmable temperature gradient range of up to 20°C enables the optimization not only of the annealing temperature, but also of all temperature steps of a PCR protocol for even the most demanding applications. SteadySlope technology ensures optimal, constant heating and cooling rates, which means that results of gradient experiments can be transferred simply and precisely to routine applications.The entire Mastercycler family incorporates the personal card system: chip-based memory cards that enable a simple and reliable transfer of your individual protocols between different Mastercycler workstations and ensure unlimited memory expansion.The universal block incorporates an expansive range of different tube types in both the Mastercycler and Mastercycler gradient: use 96 x 0.2 ml tubes, 77 x 0.5 ml tubes or one multiwell PCR plate—without having to change the block. Eppendorf #: 950010045. Gradient block1: up to 20°C gradient span is possible at any step of the PCR program. Universal Block2. Utmost flexibility, simple programming: gradient function, adjustable ramp rates, time and temperature increments, incubation mode, in situ mode, link function, pause function.